Effect of DIM on the regulation of smooth muscle gene expression.
A. VSMCs preincubated for 2 h with DIM and then stimulated with PDGF-BB (20 ng/ml) for 48 h. The protein levels of SM α-actin, SM22α, and desmin were determined with western blot analysis and quantified by densitometry. B. Bar graphs showing the quantification of western blots. The results are expressed as a percentage of control (#P<0.05 versus control group; *P<0.05 versus PDGF alone; n = 3).