Effect of AmpliconDuo filtering on chimeras for prokaryotic sample Pro2.

Chimeras defined by being recognized by UCHIME in de novo mode with score ≥ 1. Top: Frequency of chimeras in branches A, B of split sample as function of their read numbers, before (dashed lines) and after (solid lines) application of AmpliconDuo filter. Bottom: Fraction of chimeras passing the AmpliconDuo filter (f_filtered/f_unfiltered) for read numbers 1 to 20 in both branches A, B, and corresponding prediction P(r_iA, r_iB ≥ 1) using the Poisson model in Eq (8) with λ_i = 1, 2, …, 20.