Figure_3.tif (1.36 MB)

Edg-1 is involved in RTEF-1 driven angiogenesis in endothelial cells.

figure

posted on 2014-02-10, 03:22 authored by Ping He, Melissa J. Philbrick, Xiaojin An, Jiaping Wu, Angela F. Messmer-Blust, Jian Li

A. Edg-1 expression was knocked down by Edg-1 siRNA. (** = p<0.01, vs control siRNA treated RTEF-1 o/e HMEC-1) B. Edg-1 siRNA inhibited RTEF-1 driven endothelial cell aggregation. (# = p<0.05, vs negative control siRNA treated control HMEC-1; ** = p<0.01, vs negative control siRNA treated RTEF-1 o/e HMEC-1; ## = p<0.01, vs negative control siRNA treated control HMEC-1; Triangle: aggregation area; Arrow: branch) C. Edg-1 siRNA inhibited RTEF-1 driven endothelial cell migration. (# = p<0.05, vs negative control siRNA treated control HMEC-1; * = p<0.05, vs negative control siRNA treated RTEF-1 o/e HMEC-1) D. Treatment with Edg-1 siRNA attenuated cell growth in RTEF-1 o/e HMEC-1 (* = p<0.05; ** = p<0.01, vs negative control siRNA). The results were quantified based on three experiments and are presented as mean±S.D.