Edg-1 is involved in RTEF-1 driven angiogenesis in endothelial cells.

<p><b>A.</b> Edg-1 expression was knocked down by Edg-1 siRNA. (** = <i>p</i><0.01, <i>vs</i> control siRNA treated RTEF-1 o/e HMEC-1) <b>B.</b> Edg-1 siRNA inhibited RTEF-1 driven endothelial cell aggregation. (# = <i>p</i><0.05, <i>vs</i> negative control siRNA treated control HMEC-1; ** = <i>p</i><0.01, <i>vs</i> negative control siRNA treated RTEF-1 o/e HMEC-1; ## = <i>p</i><0.01, <i>vs</i> negative control siRNA treated control HMEC-1; Triangle: aggregation area; Arrow: branch) <b>C.</b> Edg-1 siRNA inhibited RTEF-1 driven endothelial cell migration. (# = <i>p</i><0.05, <i>vs</i> negative control siRNA treated control HMEC-1; * = <i>p</i><0.05, <i>vs</i> negative control siRNA treated RTEF-1 o/e HMEC-1) <b>D.</b> Treatment with Edg-1 siRNA attenuated cell growth in RTEF-1 o/e HMEC-1 (* = <i>p</i><0.05; ** = <i>p</i><0.01, <i>vs</i> negative control siRNA). The results were quantified based on three experiments and are presented as mean±S.D.</p>