ERS inhibits insulin receptor signaling in primary rat hippocampal and cortical neurons.

(A–B) Western blot analysis of the effect of 4 h tunicamycin (Tm) treatment on p-IRS-1, IRS-1, p-Akt, and Akt levels in primary rat hippocampal (A) and cortical neurons (B). (C–D) Western blot analysis of the effect of 4 h Tm treatment on p-JNK, JNK, p-IRS-1, IRS-1, p-Akt, and Akt levels in primary rat hippocampal (C) and cortical neurons (D) transfected with IRE1α siRNA. (E–F) Western blot analysis of the effect of 4 h Tm treatment on p-IRS-1, IRS-1, p-Akt, and Akt levels in primary rat hippocampal (E) and cortical neurons (F) transfected with JNK siRNA. Statistical analysis is shown in S1 Fig. Blots were digitized and bands quantified using an image analysis system.