EM analysis of 293T cells transfected with WT pNL4-3 or the indicated mutant derivatives and treated without (no inhibitor) or with 5 µM PF-46396.

Note highly defective EM morphology and accumulation of Gag at the plasma membrane for the mutants in the absence of PF-46396, and the rescue of assembly/release in the presence of compound. Scale bars: a–d, f–h, j–p = 100 nm; e, i = 500 nm.