ELISPOT detection of in vitro IFN-γ production by splenocytes of vaccinated mice.
Splenocytes from two spleens were pooled for a total of three samples per immunization group and analyzed by ELISPOT. (A) Numbers of spleen cells targeting HEV and (B) numbers of spleen cells targeting HAV were detected in the mouse groups immunized as indicated in Fig 2. Error bars represent the SEM. *, P<0.05, **, P<0.01, ***, P<0.001; ns, no significant difference.