Dve expression during accessory gland development.

(A–D) Dve protein expression. (E–H) dve enhancer activity (w; UAS-GFP.nls8/+; dG35A/+). (I–L) Prd protein expression. (A, E, and I) At the third-larval instar, male genital discs do not express Dve and Prd in accessory gland precursor cells (outlined). (B, F, and J) Accessory gland (AG) primordia at 24 hr APF start to express Dve (B) and Prd (J) (arrowheads). The shape of genital disc is outlined, and arrows indicate the seminal vesicle (SV). Accessory glands at 72 hr APF (C, G, and K) and of 1-day old adults (D, H, and L). Dve is expressed strongly in secondary cells (arrows in C, D) and weakly in pupal main cells (arrowhead in C). Dve expression in the adult main cells is undetectable (open arrowhead in D). The dve enhancer activity is nearly identical to the endogenous Dve protein expression (E–G), although the weak activity in main cells is detectable in the adult stage (arrowhead in H). Prd expression is detected in both types of cell from pupal to adult stages (K and L). (M and N) prd mutant clones of 4–6 days old adults are labeled with the absence of GFP expression (green), and are outlined with broken lines. Dve expression (red) is strongly derepressed in prd mutant main cells, which are mononucleate (M), whereas binucleation and Dve expression are unaffected in a prd mutant secondary cell (N). Nuclei (DNA) and cell membrane are labeled with TO-PRO3 and anti-Spectrin antibody, respectively (blue). Single channel images are shown in M′-M″′ and N′-N″′.