Duplication and divergence of PABPs has occurred on multiple occasions during eukaryote evolution. A)

Phylogenetic reconstruction of PABP evolutionary history using predicted protein sequences from four eukaryotic supergroups [78]. Phylogenetic analysis was performed with MrBayes, RAxML and PhyML locally, using the WAG model for ML and mixed parameters for MrBayes [79], [80], [81]. The tree was generated using the MrBayes topology. Numbers indicate Baysian posterior probabilities and bootstrap support for PhyML and RAxML respectively; a dash indicates a feature not reconstructed under a specific algorithm. Leaves are colorized according to supergroup: green for Viridiplantae, light blue for Opistokhonta, orange for Chromalveolates and purple for Excavata. Taxa are indicated by a two letter code based on Linnean nomenclature; Hs; Homo sapiens, Dm; Drosophila melanogaster, Ce; Caenorhabditis elegans, DR; Danio rerio, Nv; Nematostella vectensis, Cn; Cryptococcus neoformans, Sp; Schizosaccharomyces pombe, An; Aspergillus nidulans, Ro; Rhizopus oryzae, At; Arabidopsis thaliana, Ot; Oryza sativa, Pt; Populus trichocarpa, Pp; Physcomitrella patens, Ot; Ostreococcus tauri, Pf; Plasmodium falciparum, Tg; Toxoplasma gondii, Cp; Cryptosporidium parvum, Tp; Theileria parva, Pr; Phytophthora ramorum, Tb; Trypanosoma brucei and Lm; Leishmania major. Note that he apparent split of the Viridiplantae clade is an artefact of the manner in which the tree has been drawn, and that monophyly is well supported. B) Phylogenetic reconstruction of PABPs within the Excavata prepared as above. Taxa are indicated by a three letter code based on Linnean nomenclature: Tva, Trichomonas vaginalis; Egr, Euglena gracilis; Tbo, Trypanoplasma borrelli; Bsa, Bodo saltans; Pse, Phytomonas serpens; Pda, Phytomomas davidii; Lb, Leishmania braziliensis; Lin, Leishmania infantum; Lma, Leishmania major; Tbr, Trypanosoma brucei; Tco, Trypanosoma congolense; Tcr, Trypanosoma cruzi; Tth, Trypanosoma theileri; Tgr, Trypanosoma grayi and Tca, Trypanosoma carassii.