Dullard does not affect BMP4 signalling.

<p>(<b>A–A′′</b>) The expression pattern of p-Smads in the proximal epiblast and adjacent extraembryonic ectoderm of the E6.75 <i>Dullard</i><sup>−/−</sup> embryo was similar to that in <i>Dullard</i><sup> +/−</sup> embryos (<b>A</b>, whole embryo bright-field view; <b>A′</b>, immunofluorescence image; <b>A′′</b>, merged image; left pair, <i>Dullard</i><sup>−/−</sup> embryos; right pair, <i>Dullard</i><sup> +/−</sup> embryos). (<b>B, C</b>) Confocal images of immunostaining of p-Smads in <i>Dullard</i><sup>−/−</sup> and <i>Dullard</i><sup> +/−</sup> embryos, respectively. (<b>D–I′</b>) Formation of Dpp3/Pgc7/Stella-positive PGCs was unaffected by the combined loss-of-function of <i>Dullard</i> and <i>Bmp4</i>. (<b>D–F′</b>) E7.75 compound heterozygous <i>Dullard; Bmp4</i> embryos contained similar numbers of PGCs as those in <i>Dullard<sup>+/−</sup></i> and <i>Bmp4<sup>+/−</sup></i> embryos. (<b>G–I′</b>) Loss of <i>Bmp4</i> activity in addition to complete loss of <i>Dullard</i> function had no effect on the PGC phenotype of E7.75 <i>Dullard</i><sup>−/−</sup> embryos. (<b>D–I)</b> Whole embryo bright-field image; (<b>D–G</b>, posterior view; <b>H, I,</b> lateral views). (<b>D′–I′</b>) Dppa3/Pgc7/Stella immunostaining of the embryo in panels <b>D–I</b>. Scale bars = 100 µm (<b>A–A′</b>′), 20 µm (<b>B, C</b>) and 50 µm (<b>D–I′</b>).</p>

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