Figure_7.tif (2.14 MB)
Downstream signaling in doxazosin-induced apoptosis.
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posted on 2014-02-06, 10:45 authored by Ingo Staudacher, Julian Jehle, Kathrin Staudacher, Hans-Werner Pledl, Dieter Lemke, Patrick A. Schweizer, Rüdiger Becker, Hugo A. Katus, Dierk ThomasDoxazosin treatment induced cleavage of effector caspases 7 (B) and 3 (D), associated with unaltered expression of pre-processed caspase 3 and 7 levels in LNT-229 cells (A, C). (E) Enhanced cleavage (inactivation) of poly-ADP-ribose-polymerase (PARP) was observed with increasing concentrations of doxazosin, representing a characteristic apoptotic step. (F) Autophagy-associated phagocytosis of dead cells is indicated by increased levels of the lower migrating form of microtubule-associated protein 1 light chain 3 (LC3). Typical Western blots and mean (± SEM) optical densities normalized to drug-free conditions are shown (n = 3 independent assays; *p<0.05; **p<0.01; GAPDH, glyceraldehyde-3-phosphate dehydrogenase).
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Anatomy and physiologyNeurological systemNerve tissueelectrophysiologygeneticsCancer geneticsMolecular cell biologySignal transductionSignaling in cellular processesAntiapoptotic signalingApoptotic signalingSignaling in selected disciplinesOncogenic signalingCell deathMembranes and sortingcardiovasculararrhythmiasCardiovascular pharmacologyHeart failureDrugs and devicesDrug research and developmentdrug discoveryoncologyBasic cancer researchTumor physiologydoxazosin-induced
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