Diversity of ACME and SCC<i>mec</i> among <i>S. epidermidis</i>.

1<p>“+” methicillin-resistant <i>S. epidermidis</i>; “−”, methicillin-susceptible <i>S. epidermidis</i>; <sup>2</sup>CC, clonal complex, as previously defined by eBURST analysis <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007722#pone.0007722-Miragaia2" target="_blank">[15]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007722#pone.0007722-Miragaia3" target="_blank">[16]</a>; S, singleton; <sup>3</sup>MLST, multilocus sequence typing <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007722#pone.0007722-Thomas1" target="_blank">[14]</a>; 7-loci allelic profile listed in parenthesis (<i>arcC-aroE-gtr-mutS-pyrR-tpiA-yqiL</i>); <sup>4</sup>ACME PCR scan method <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007722#pone.0007722-Diep2" target="_blank">[5]</a> defines genetic variants within 3 ACME allotypes: ACME-I contains <i>arc</i> and <i>opp-3</i> gene clusters; ACME-II contains <i>arc</i> but not <i>opp-3</i>; and ACME-III contains <i>opp-3</i> but not <i>arc</i>. ACME-neg is negative for both <i>arc</i> and <i>opp-3</i>. Amplicons from the ACME PCR scan for 5 underlined ACME-I.02 variants were used for DNA sequencing and construction of 25-kb contigs. <sup>5</sup>NH, no hybridization with <i>arcCB</i> probe; <i>ClaI</i>-<i>arcCB</i> banding patterns shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007722#pone-0007722-g003" target="_blank">Figure 3</a>; <sup>6</sup>SCC<i>mec</i> typing to identify class A, B, C, and other non-typeable (NT) <i>mec</i> gene complex, and 5 types of <i>ccr</i> gene complex, ccrAB1, ccrAB2, ccrAB3, ccrAB4, ccrC. SCC<i>mec</i> type I contains B/ccrAB1; type II A/ccrAB2; type III A/ccrAB3; type IV B/ccrAB2; and type V C/ccrC.</p>