Distribution of fibronectin and actin inside scaffold pores.

Actin (green) and fibronectin (red) intensity distribution from the pore center to the pore wall as extracted from histological images (Fig. 6a, n_pores = 4 for each category). From the maximum projection of the z-stack and the contoured pore, the Pearson coefficient for green and red channel colocalization was calculated. The whole intensity data range for each channel (0–255) was considered here (Fig. 6b, n = 5 for each category).