Digestion of 1 µg mutated phagemid DNA library pools with HindIII and SacII.

(A) Kunkel −UDG, (B) Kunkel + UDG, (C) Kunkel −UDG +RCA, (D) Kunkel +UDG +RCA with exoresistant random primers, (E) Kunkel +UDG +RCA with normal random primers, (−) SacII resistant control, (+) SacII sensitive control, (L) 1 kb Fermentas DNA Ladder.