Figure_2.tif (6.93 MB)
Differentiation of hNPs into neurons after 3 weeks of maturation.
figure
posted on 2013-02-20, 17:49 authored by Gabriel Nistor, Monica M. Siegenthaler, Stephane N. Poirier, Sharyn Rossi, Aleksandra J. Poole, Maura E. Charlton, John D. McNeish, Chris N. Airriess, Hans S. KeirsteadA) hNP-derivates displayed a highly branched morphology, consistent with a neuronal phenotype. B) Immunolabeling with TUJ1 (green) and GFAP (red) revealed a high purity neuronal culture with few astrocytes. C) Cells were immunopositive for Tau, a microtubule associated protein, consistent with neuronal differentiation. D) Cells were immunopositive for MAP2, a microtubule associated protein, consistent with neuronal differentiation.