Differences in α-dystrobrevin splice variants between cardiac and skeletal muscle DAPC.

<p>(<b>A</b>) Western blot analysis of α-dystrobrevins in mouse cardiac (C) and skeletal (S) muscle total protein lysates, DYS-IPs and IgG-IPs. α3-dystrobrevin associates with dystrophin in the heart. Fold differences in α-dystrobrevin abundance in cardiac vs. skeletal muscle DYS-IPs relative to dystrophin are shown (averages ±SD, N = 3). (<b>B</b>) Immunolabeling of wild type cardiac sections for α1 and α2-dystrobrevins. Scale bar: 50 µm. (<b>C</b>) Immunolabeling of <i>mdx</i> cardiac tissue section for β-sarcoglycan. Scale bar: 50 µm. (<b>D</b>) Western blot analysis of α-dystrobrevins in heart protein lysates from wild type (WT) and <i>mdx</i> mice. Fold differences in α-dystrobrevin abundance in WT vs. mdx cardiac lysates relative to GAPDH are shown (averages ±SD, N = 3) (<b>E</b>) Western blot analysis of α-dystrobrevins in DYS-IPs from human (H) and mouse (M) cardiac samples. Additional α-dystrobrevin isoforms (arrow heads) are detected in human cardiac lysates and DYS-IP.</p>