Developmentally regulated expression of testosterone-responsive miRNAs.

(A) Diagram of the mouse X chromosome showing location of two miRNA clusters. Cluster 1 includes six miRNAs (panels b–g) and cluster 2 includes two miRNAs (panels h and i). (B) Real-time RT-PCR analysis of selected miRNA (panels a–h) expression in RNA isolated from purified Sertoli cells from mice of ages shown. Sertoli cells were pooled from seven mice for 7 days (n = 2 different experiments) and from six mice for each of 13 days (n = 2 different experiments), 21 days (n = 3 different experiments), and 60 days (n = 3 different experiments). All values were normalized against 5S levels. MiRNAs in cluster 2 exhibit quantitative as well as temporal collinear expression pattern.