Determining optimal read depth in SB-induced tumors.
(A) An experiment was performed to simulate various read depths in 30 Vav-SB (left) and 32 CD4-SB (right) tumors. The average number of clonal transposon insertion sites was determined from 20 independent iterations of each read depth simulation. (B) The consistency of transposon insertion site identification varied with sequence depth. For example, only 10% of transposon insertion sites were identified in 100% simulations at a simulated read depth of 1,000.