Figure_2.tif (107.38 kB)
Detergent screening of mAQP4 M23 expressed in the D-CF mode.
figure
posted on 2013-02-20, 23:08 authored by Lei Kai, Ralf Kaldenhoff, Jiazhang Lian, Xiangcheng Zhu, Volker Dötsch, Frank Bernhard, Peilin Cen, Zhinan XuA: RM samples of 2 µl were analyzed by 16% SDS-PAGE and immunoblotted using anti-His antibodies. B: Solubility of D-CF expressed mAQP4 M23 in presence of 0.2% Brij-35, 0.4% Digitonin, 0.1% Triton X-100, and 0.05% Tyloxapol. Control is P-CF expressed mAQP4 M23. S, supernatant; P, pellet.
