Detection of pullulanase activity in GBS total extracts.
Bacteria grown in a complex medium supplemented with the indicated sugars were used to prepare total extracts. Bacterial extracts were then incubated with pullulan and pullulanase enzymatic activity measured by DNS acid assay. The asterisks indicate a significant difference between the activity of the wild type strain versus the mutant strain derived extracts (p<0,01). The data are the mean of 3 independent experiments ± SD.