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Detection of native CfNOS activity from haemocyte lysates.

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posted on 2013-07-26, 02:38 authored by Qiufen Jiang, Zhi Zhou, Leilei Wang, Lingling Wang, Feng Yue, Jingjing Wang, Linsheng Song

The protein of CfNOS was immunoprecipitated by polyclonal antibody of rCfNOS, and incubated with the elution buffer, Spd (0.01 mmol L-1), L-NAME (0.01 mmol L-1), and SMT (0.01 mmol L-1), respectively. CfNOS activity was detected by measuring the NO production using L-Arginine as the substrate. The elution buffer was used as blank. Results were expressed as unit activity per milligram protein (U mgprot-1). Each value was shown as mean ± SD (N = 3) and bars with different letters were significantly different (P < 0.05).

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