Detection of native CfNOS activity from haemocyte lysates.
The protein of CfNOS was immunoprecipitated by polyclonal antibody of rCfNOS, and incubated with the elution buffer, Spd (0.01 mmol L-1), L-NAME (0.01 mmol L-1), and SMT (0.01 mmol L-1), respectively. CfNOS activity was detected by measuring the NO production using L-Arginine as the substrate. The elution buffer was used as blank. Results were expressed as unit activity per milligram protein (U mgprot-1). Each value was shown as mean ± SD (N = 3) and bars with different letters were significantly different (P < 0.05).