Detection of cytokines on membrane antibody arrays by chemiluminescence.

Duplicate spots in the following locations represent each cytokine. See Figure 1. The average net light intensity for each pair of cytokine spots detected based on ray-scale levels using US NIH Image software ver 1.66. Cytokines names: Neutrophil chemotactic cytokine 2 and 3 (CINC-2 and CINC-3), ciliary neurotrophic factor (CNFT), monocyte chemotactic protein-1 (MCP-1), inflammatory protein macrophage-3 alpha (MIP-3 alpha), nerve growth factor beta (beta-NGF), tissue inhibitor of metalloproteinase-1 (TIMP-1) and vascular endothelial growth factor (VEGF), granulocyte colony stimulating factor, macrophage (GM-CSF), interferon gamma (INF-γ), interleukin 1 alpha and beta (IL-1α, IL-1β), interleukin 4, 6 and 10 (IL-4, IL-6, IL-10), lipopolysaccharide induced CXC chemokine (LIX or CXCL5), leptin, tumor necrosis factor alpha (TNF-α).