Detection of cytokines on membrane antibody arrays by chemiluminescence.

<p>Duplicate spots in the following locations represent each cytokine. See <a href="" target="_blank">Figure 1</a>. The average net light intensity for each pair of cytokine spots detected based on ray-scale levels using US NIH Image software ver 1.66. Cytokines names: Neutrophil chemotactic cytokine 2 and 3 (CINC-2 and CINC-3), ciliary neurotrophic factor (CNFT), monocyte chemotactic protein-1 (MCP-1), inflammatory protein macrophage-3 alpha (MIP-3 alpha), nerve growth factor beta (beta-NGF), tissue inhibitor of metalloproteinase-1 (TIMP-1) and vascular endothelial growth factor (VEGF), granulocyte colony stimulating factor, macrophage (GM-CSF), interferon gamma (INF-γ), interleukin 1 alpha and beta (IL-1α, IL-1β), interleukin 4, 6 and 10 (IL-4, IL-6, IL-10), lipopolysaccharide induced CXC chemokine (LIX or CXCL5), leptin, tumor necrosis factor alpha (TNF-α).</p>