Detection of Ply specific CD127^low/- FoxP3⁺ CD4⁺ Treg cells in CD25 enriched tonsil and blood MNC.

CD25 enriched MNC were stained with anti- CD127 and anti FoxP3 to allow identification of Tregs and with (a) strepavidin-PE, (b) negative control Ply tetramer-PE and (c) Ply tetramer-PE and analysed by FACS. A typical example of a FACS plot is shown. While the CD127^low/- FoxP3⁺ CD4⁺ Treg cells show low level staining at 0.0% with strepavidn-PE (a) and negative control Ply-tetramer at 0.05% (b), a significantly higher percentage of Treg cells are bound by Ply-tetramers at 1.96%(c).