Design and Construction of NBPs harboring λ ZAP-CMV-apoptin expression plasmid.

<p>(<b>A</b>) The expression vector was subsequently inserted into the λ phage, generating the recombinant NBPs, (<b>B</b>) Different cell types were infected with the indicated recombinant λ NBPs at an MOI of 10 PFU/cell and apoptin transcription was analyzed by RT-PCR, (<b>C</b>) RT-PCR result for λ ZAP-CMV vector treated cells that have no expression of apoptin, (<b>D</b>) Western blot analysis to detect apoptin protein from cells supernatants and lysates. To analyze apoptin expression, the cells were infected with the indicated recombinant NBPs. Purified apoptin was used as a positive control and CAV infected BT-474 cell was used as a negative control.</p>

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