Delivery of S14G-HN to brain by intranasal administration.

3xTg-AD mice (13 month-old) were intranasally administrated with S14G-HN (B) or vehicle (A), and sacrificed after 1 hour of administration. Brains were fixed with 4% paraformaldehyde and 30 µm sagittal sections of brains were subjected to immunostaining using anti-HN antibody (P04) and biotin-conjugated secondary antibody followed by visualization with avidin-fluorescein. ACB, Nucleus accumbens; AON, Anterior olfactory nucleus; NBD, Diagnal band nucleus; OT, Olfactory tubercle; SI, Substantia innominata. Bar = 500 µm.