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Deletion of Raptor in preosteoblasts reduced matrix synthesis and mineralization.

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posted on 2015-06-19, 03:53 authored by Jianquan Chen, Fanxin Long

(A-B) Alkaline phosphatase (AP) staining of wildtype (Ad-GFP)(A) versus Raptor-deficient (Ad-Cre)(B) calvarial cells after 7 days (D7) of osteogenic differentiation. (C-F)Von Kossastaining of wildtype (Ad-GFP)(C, E) versus Raptor-deficient (Ad-Cre)(D, F) calvarial cells after 14 days (D14) of osteogenic differentiation. Panels C-D showed lower magnification images; Panels E-F showed higher magnification images. (G) Quantification of bone nodules detected by Von Kossa staining. n = 3; *:P<0.05. (H) ELISA analysis of P1NP levels in wildtype (Ad-GFP) versus Raptor-deficient (Ad-Cre) calvarial cells after 14 days (D14) of osteogenic differentiation. n = 3; *:P<0.05.

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