Deletion of <i>Raptor</i> in preosteoblasts reduced matrix synthesis and mineralization.

2015-06-19T03:53:29Z (GMT) by Jianquan Chen Fanxin Long
<p>(A-B) Alkaline phosphatase (AP) staining of wildtype (Ad-GFP)(A) versus <i>Raptor</i>-deficient (Ad-Cre)(B) calvarial cells after 7 days (D7) of osteogenic differentiation. (C-F)Von Kossastaining of wildtype (Ad-GFP)(C, E) versus <i>Raptor</i>-deficient (Ad-Cre)(D, F) calvarial cells after 14 days (D14) of osteogenic differentiation. Panels C-D showed lower magnification images; Panels E-F showed higher magnification images. (G) Quantification of bone nodules detected by Von Kossa staining. n = 3; *:P<0.05. (H) ELISA analysis of P1NP levels in wildtype (Ad-GFP) versus <i>Raptor</i>-deficient (Ad-Cre) calvarial cells after 14 days (D14) of osteogenic differentiation. n = 3; *:P<0.05.</p>