Defective cleavage at poly(A) sites in muscles expressing PABPN1-17ala and in the Pabp2 mutant.

A) Schematic representation of primers (arrows) used to quantify uncleaved pre-mRNA. B) Quantification of uncleaved pre-mRNAs in control and PABPN1-17ala-expressing thoraxes at day 2, using RT-qPCR. Uncleaved RNAs were normalized to Cpr100A uncleaved RNA. Cpr100A is expressed in the cuticle and its expression remains unaffected by expression of PABPN1-17ala in muscles. Means of three biological replicates quantified three times. For (B) and (C), error bars represent standard deviation. * p-value <0.05, ** p-value <0.01, *** p-value <0.001, ns: not significant, using the Student’s t-Test. C) Quantification of uncleaved pre-mRNAs in control (w¹¹¹⁸) and Pabp2 (Pabp2⁵⁵/Df(2R)CA53) mutant first instar larvae, using RT-qPCR. Uncleaved RNAs were normalized to RpS6 uncleaved RNA. The levels of RpS6 uncleaved RNA normalized to sop mRNA were unaffected in Pabp2 mutant larvae (right panel). Means of three biological replicates quantified three times.