Defective cleavage at poly(A) sites in muscles expressing PABPN1-17ala and in the <i>Pabp2</i> mutant.

<p>A) Schematic representation of primers (arrows) used to quantify uncleaved pre-mRNA. B) Quantification of uncleaved pre-mRNAs in control and PABPN1-17ala-expressing thoraxes at day 2, using RT-qPCR. Uncleaved RNAs were normalized to <i>Cpr100A</i> uncleaved RNA. <i>Cpr100A</i> is expressed in the cuticle and its expression remains unaffected by expression of PABPN1-17ala in muscles. Means of three biological replicates quantified three times. For (B) and (C), error bars represent standard deviation. * <i>p</i>-value <0.05, ** <i>p</i>-value <0.01, *** <i>p</i>-value <0.001, ns: not significant, using the Student’s t-Test. C) Quantification of uncleaved pre-mRNAs in control (<i>w</i><sup><i>1118</i></sup>) and <i>Pabp2</i> (<i>Pabp2</i><sup><i>55</i></sup><i>/Df(2R)CA53</i>) mutant first instar larvae, using RT-qPCR. Uncleaved RNAs were normalized to <i>RpS6</i> uncleaved RNA. The levels of <i>RpS6</i> uncleaved RNA normalized to <i>sop</i> mRNA were unaffected in <i>Pabp2</i> mutant larvae (right panel). Means of three biological replicates quantified three times.</p>