DSB-2 and SUN-1 S8P persist when DSB formation is defective.

<p>(A) and (B) Immunofluorescence images of gonads from the distal pre-meiotic region to end of pachytene, stained with DAPI and antibodies that recognize DSB-2 and SUN-1 S8P. The zone of DSB-2 and SUN-1 S8P-positive nuclei is extended in both <i>spo-11</i> (A) and <i>him-17</i> (B) mutants, which are defective in DSB formation. (C) Close-up images of fields of nuclei in early pachytene, as outlined in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003674#pgen-1003674-g003" target="_blank">Figure 3A</a> and (A), (B) above. WT as well as <i>spo-11</i> nuclei show bright patches of DSB-2 staining, whereas <i>him-17</i> nuclei do not. Scale bar, 15 µm.</p>