DSB-2 and SUN-1 S8P persist when DSB formation is defective.

(A) and (B) Immunofluorescence images of gonads from the distal pre-meiotic region to end of pachytene, stained with DAPI and antibodies that recognize DSB-2 and SUN-1 S8P. The zone of DSB-2 and SUN-1 S8P-positive nuclei is extended in both spo-11 (A) and him-17 (B) mutants, which are defective in DSB formation. (C) Close-up images of fields of nuclei in early pachytene, as outlined in Figure 3A and (A), (B) above. WT as well as spo-11 nuclei show bright patches of DSB-2 staining, whereas him-17 nuclei do not. Scale bar, 15 µm.