DNMAML expression does not inhibit oncogenic K-ras mediated ADM in vitro.
(A) Pancreatic explants from Pdx1-Cre;LSL-KrasG12D mice at Day 2, infected with control adenovirus (Ad-empty) or Adenovirus expressing DNMAML (Ad-dnMAML/GFP). Representative brightfield images of ductal cysts shown. Scale bar, 100 µm. (B) Pdx1-Cre;LSL-KrasG12D acinar explants expressing DNMAML-GFP form cytokeratin positive cysts. Explants were stained with antibodies against GFP (green) and pan-cytokeratin (red). Nuclei are counterstained with Hoechst (blue). Images are representative of 3 separate experiments. Scale bar, 20 µm. (C) DNMAML expression inhibits Notch signaling in Pdx1-Cre;LSL-KrasG12D acinar explants. Explants were stained with antibodies against GFP (green) and Hes1 (red). Nuclei are counterstained with Hoechst (blue). Scale bar, 20 µm.
