DLK1 is expressed in specific nuclei of the hypothalamus.

A) Immunohistochemistry using the C-19 antibody was performed on free-floating coronal brain sections from adult male mice as described in materials and methods (2,5× objective; Bregma −0.82 mm at the hypothalamic level according to the mouse brain atlas [21]. B) Bregma −2.06 mm C) Higher magnification of the paraventricular nucleus (PVN), suprachiasmatic nucleus (SCN), supraoptic nucleus (SON) arcuate nucleus (ARC), and lateral hypothalamic nucleus (LH) (20× objective; scale bar: 80 µm).