DG GC of tet-hTau singly transgenic mice express hTau but not abnormal tau.

(A–P) Brain sections from tet-hTau singly transgenic mice of different ages were immunostained with the hTau-specific antibody HT7 (A–H) or with antibodies that recognize misfolded (MC1; I–L) or abnormally phosphorylated (CP-13; M–P) tau. (A–D) Low power images show prominent expression of hTau in GC axons (mossy fibers, arrowheads) at all ages. (E–H) Cell bodies of GC were also variably immunoreactive for hTau at all ages examined. (I–P) GC cell bodies of tet-hTau singly transgenic mice did not stain with MC-1 (I–L) or CP-13 (M–P). However, we did observe MC-1 positive MFs in tet-hTau singly transgenic mice at all ages (data not shown). Scale bars: 500 µm (A–D) and 50 µm (E–P).