Cytopathic effect in Vero cells infected with avian paramyxoviruses (APMVs).

Vero cells in six-well plates were infected with each of APMVs at a multiplicity of infection (MOI) of 0.1 PFU/cell and incubated for 48 h. Exogenous protease was provided in the case of APMV-3, -8, and -9 (Materials and Methods). The viral plaques in the infected cells were visualized by immunoperoxidase staining using polyclonal antibodies raised against the N protein of the respective APMV.