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Cytopathic effect in Vero cells infected with avian paramyxoviruses (APMVs).

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posted on 2013-02-20, 05:39 authored by Shin-Hee Kim, Sa Xiao, Heather Shive, Peter L. Collins, Siba K. Samal

Vero cells in six-well plates were infected with each of APMVs at a multiplicity of infection (MOI) of 0.1 PFU/cell and incubated for 48 h. Exogenous protease was provided in the case of APMV-3, -8, and -9 (Materials and Methods). The viral plaques in the infected cells were visualized by immunoperoxidase staining using polyclonal antibodies raised against the N protein of the respective APMV.

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