Copy Number Variation (CNV) indicates that BRAF is amplified and bisected in resistant clones.

<p>Relative to GTL16, CNV analysis identifies a region of increased copy number at 7q34, with a breakpoint within the <i>BRAF</i> locus in GTL16R1 and GTL16R3. In conjunction with Affymetrix expression analysis, genes within the amplified regions are coordinately over-expressed (indicated in red) normalized to GTL16. Expression of the BRAF transcript is increased over GTL16.</p>