Constructs and clones used in the study.

a<p>pME22 was modified from pBBE22 by removing the <i>bbe22</i> copy from the <i>Acc</i>65I site to the <i>Aat</i>II in order to make the <i>Acc</i>65I site available for cloning <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053212#pone.0053212-Shi1" target="_blank">[19]</a>.</p>