Fig_1.tif (985.37 kB)
Construction, expression and purification of scFv-LT.
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posted on 2015-07-08, 03:23 authored by Christiane Y. Ozaki, Caio R. F. Silveira, Fernanda B. Andrade, Roberto Nepomuceno, Anderson Silva, Danielle D. Munhoz, Bruno B. Yamamoto, Daniela Luz, Patrícia A. E. Abreu, Denise S. P. Q. Horton, Waldir P. Elias, Oscar H. P. Ramos, Roxane M. F. Piazza(A) scFv-LT amino acids sequence: VH—Heavy chain variable domain, L—Linker and VL—Light chain variable domain sequence. CDRs are highlighted in yellow for VH and in blue for VL. (B) SDS-PAGE analysis of scFv-LT recombinant antibody expression and purification. Lanes: 1. C43(DE3) non-induced fraction; 2. C43(DE3) induced fraction; 3. Insoluble fraction; 4. Purified scFv-LT.
