Conformational fingerprinting of mutant ACE.

<p>Membrane-bound WT and mutant ACE lysates were normalized to achieve 5 mU/ml ACE activity with Z-Phe-His-Leu as substrate and incubated in microtiter plate wells covered with 16 mAbs to human ACE <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010438#pone.0010438-Naperova1" target="_blank">[16]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010438#pone.0010438-Danilov4" target="_blank">[30]</a> via goat-anti-mouse IgG. Precipitated ACE activity was quantified by fluorimetric plate precipitation assay <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010438#pone.0010438-Danilov4" target="_blank">[30]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010438#pone.0010438-Danilov6" target="_blank">[54]</a>. Data (mean ± SD of 6–8 independent experiments in duplicate) are expressed as ratio of ACE activity precipitated by mAbs from mutant ACE to that of WT ACE. * p<0.05 vs. WT ACE.</p>