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Confirmation of HTS candidates by FP competition assay.

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posted on 2015-09-14, 02:46 authored by Xinlu Li, Marie Ménade, Guennadi Kozlov, Zheping Hu, Zheng Dai, Peter S. McPherson, Bernard Brais, Kalle Gehring

A and B. 5 nM F12-ATP and 200 nM GST-HEPN were titrated with the hits to confirm their binding to GST-HEPN. C. Affinities of the compounds for HEPN were estimated from the competition assays and summarized along with their chemical structures.

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