Concentration- and time-dependent induction of HO-1 and NQO1 by hemin in endothelial cells.

(A) Western blot analysis confirming concentration dependent induction of HO-1 and NQO1 in PMVECs and PAECs treated with hemin for 7 days. Blots were probed for EF-1αto control protein loading. (B, C) Quantification of HO-1 and NQO1 protein expression in PAECs, PMVECs, BMVECs and DMVECs treated with hemin and vehicle, assayed by western blot analysis. Data shown is mean fold change in protein level as arbitrary units relative to the EF-1α-normalized expression in vehicle treated cells (n = 9). (D) Quantification of western blot showing variable timing of HO-1 and NQO1 induction by hemin (5 µM) in PAECs (n = 9).