Complex force-distance curves with multiple rupture peaks detected with WT alpha-synuclein (A, B) and A30P mutant (C, D).

Red, green and blue lines on the graphs are calculated WLC curves using maxima of Gaussians determined from contour length distributions corresponding to 26, 36 and 47 nm (WT) and 22, 34 and 49 nm (A30P). E) Pie charts showing relative contribution of multiple rupture events to total rupture events: 31% (WT) and 15% (A30P).