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Complementation of the S. pombe Δcoq4, Δcoq6 and Δcoq3 strains by HsCOQ3, HsCOQ4 and HsCOQ6.

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posted on 2014-06-09, 02:43 authored by Kazuhiro Hayashi, Yuki Ogiyama, Kazumasa Yokomi, Tsuyoshi Nakagawa, Tomohiro Kaino, Makoto Kawamukai

(A, B) HPLC analyses of lipid extracts from the KH4 (Δcoq4) strain expressing S. pombe coq4 or HsCOQ4 and the KH6 (Δcoq6) strain expressing S. pombe coq6 or HsCOQ6. Growth on minimal media (EMM) plates is also shown. (C) HPLC analyses of lipid extracts from the KH3 (Δcoq3) strain expressing S. pombe coq3, HsCOQ3, or HsCOQ3 fused with a mitochondrial targeting sequence (Mt-signal).

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