Comparison of different methods for the prediction of critical residues in protein interfaces using a BID derived dataset as described in Tuncbag et al. [18].

Predictions were performed using PCRPi [7] with an expert BN trained in a Ab+ dataset that does not include the crystal structure of the c2 fragment of streptococcal protein G in complex with the Fc domain of human Ig (PDB code 1fcc).

Values were obtained running FoldX [19] with default parameters and a ddG_binding cut-off of 2.0 Kcal.mol⁻¹ (i.e. residues were considered critical if upon mutation to Ala, predicted ddG_binding≥2.0 Kcal.mol⁻¹).

Precision, recall, and F1 score values taken from Tuncbag et al. [18].