Comparison of SV40NLS peptides binding to the major (a) and minor (b) binding sites for Impα structures from different organisms.

SV40NLS peptides in complex with NcImpα (magenta), ScImpα (green), OsImpα (blue) and MmImpα (yellow) were superimposed using the Cα atoms of the peptides. Positions binding corresponding to the major (P1-P5) and minor (P1′-P4′) sites are identified along the chains (binding pocket labels were based on peptide binding with NcImpα).