Comparison of HCRSV symptoms on kenaf leaves infected with wild-type (wt) virus and its two mutants at 19 days post inoculation.

HCRSV symptoms were not observed in the mock control. HCRSV wt infected leaf displayed chlorosis. Symptoms were observed in the inoculated leaves (red circles with dotted outline) but not in the newly emerged leaves (white squares with dotted outline) of p223 (H A) and p223 (K, R A, A) mutants. Close-ups of newly emerged leaves and inoculated leaves are shown in inserts with squares and circles, respectively.