Combined inactivation of MYC and <i>K-ras<sup>G12D</sup></i> in lung tumor cells results in a shutdown of Stat3 signaling.

<p>(A) Representative phospho-Stat5 and (B) phospho-Stat3 IHC analysis demonstrates little to no levels of nuclear staining in cells following dual inactivation of MYC/<i>K-ras<sup>G12D</sup></i> (n = 3 “On” & 6 “Off”) similar to conditional <i>K-ras<sup>G12D</sup></i>–induced tumors. MYC/K-ras #2-3 represent independent inactivated tumors with no to highest amount of staining observed, respectively. IHC was performed similar to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0002125#pone-0002125-g001" target="_blank">Figure 1D</a> with stated antibodies with CMR-induced lung tumors that were activated or inactivated (2–11 weeks). Adjacent bar graph panels represent scoring of individual tumors for IHC staining: negative, low (<50% positive cells) or high (≥50% positive cells) for phospho-Stat5 and phospho-Stat3 positive tumors.</p>