Co-immunoprecipitation of SOLO and SMC1 from ovarian extracts.

2013-07-18T03:49:41Z (GMT) by Rihui Yan Bruce D. McKee
<p>Ovary lysates were prepared from <i>y w</i> and FH::SOLO (<i>{UASp-FH::SOLO}/CyO; {nos-GAL4::VP16}/TM2</i>) ovaries and used for Western blot and immunoprecipitation experiments. (A) Western blot of protein extracts from FH::SOLO and <i>y w</i> ovaries using anti-FLAG antibody. Absence of signal in the <i>y w</i> lane shows that the anti-FLAG antibody is specific. (B) Immunoprecipitation with anti-SMC1 antibody from protein extracts from FH::SOLO and <i>y w</i> ovaries, analyzed with anti-FLAG antibody. (C) Immunoprecipitation with anti-SMC1 antibody or rabbit serum (mimic control) from FH::SOLO ovary extracts, analyzed with anti-FLAG antibody. (D) Immunoprecipitation with anti-FLAG antibody or mouse serum (mimic control) from FH::SOLO ovary extracts, analyzed with anti-SMC1 antibody.</p>