Co-expression of mCherry-IncB and GFP-LC3 as a marker for autophagosomes.

<p>GFP -LC3ΔC22, which lacks the C-terminal 22 amino acids, is not correctly processed and does not localize to autophagosomes <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0063426#pone.0063426-Kabeya1" target="_blank">[34]</a> was used as a negative control. Bar = 10 µm.</p>