Clonal instability of translocations.

(A) Double peaks in the chromatogram indicate presence of C/A and C/L/A translocations in the same clone. Seven single nucleotide differences, indicated in red, distinguish the HER-II regions of ALP1 and LYP1. (B) Translocations in forty single colonies derived from unstable clone 1095 were characterized. The locations of CAN1 (black), LYP1 (green), ALP1 (red) in the parental strain are shown on the left; multiple translocations identified in clone 1095 are shown in the column “original clone”; nine different combinations (bottom row) of six different translocations (types 1–6) in the forty single colonies are schematically depicted in the right column. The three translocation types indentified in the original clone are named type 1, type 2 and type 3, indicated below the column as [1], [2], [3]. Three translocations with new breakpoints were observed, named “4”, “5” and “6”. The mixture of various translocations indentified in each clone is indicated in brackets below each column, and the number of clones with a particular translocation mixture is indicated at the bottom of each column. (C) aCGH reveals chromosome end degradation in two clones with single C/L dicentrics. Clones 608 (top) and 349J (bottom) were isolated from sgs1Δ mec3Δ and sgs1Δ rad24Δ mutants, respectively. The original CAN1 breakpoint is indicated by a vertical line.




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