Cids-2 downregulates the expression of PGL-1 in C. elegans somatic cells.

(A) Differential interference contrast (DIC, left raw) and fluorescent (right raw) images of C. elegans (bnIs1[pie-1p::gfp::pgl-1]) fed E. coli. that carry a plasmid for control, cids-2, or lin-35 RNAi. GFP signals in the head regions (white brackets) were elevated in cids-2 and lin-35 RNAi worms. (B) The bar graph shows the calculated signal intensity of control, cids-2, or lin-35 RNAi (mean ± S.D., arbitrary units). The GFP signals of cids-2 and lin-35 RNAi animals were significantly increased compared to control RNAi worms. *p<0.001, compared to control RNAi. (C) Knockdown of cids-2 led to the accumulation of the authentic pgl-1 transcript. (left) A schema of pgl-1 RT-PCR analysis. Synchronized glp-4(bn2) mutant worms at L1 stage were fed E. coli. that carried a plasmid for control, cids-2, or lin-35 RNAi and were grown to the L4 stage at the restrictive temperature (25°C). RNAs were then prepared from these L4 animals and subjected to RT-PCR. (right) RT-PCR analysis of the endogenous pgl-1 mRNA was performed with three independent samples for each of the RNAi-treated groups, and act-1 was used as a control. RT (-), no reverse transcription.