Chrysin inhibits AGS cells invasion by suppressing MMP-9.

Cells were incubated with 50 nM PMA in the presence or absence of 30 μM chrysin or 200 ng/ml MMP-9 antibody in BIOCOAT Matrigel apparatus for 24 h (A and B). Cells were incubated with 50 nM PMA in the presence of 0–50 μM chrysin (C). Cells were incubated with 50 nM PMA in the presence of 30 μM chrysin, 20 μM curcumin, and either 30 μM PD98059 (PD) or 30 μM SP600125 (SP) (D). After incubation, the cells invaded the undersurface of the chambers and were counted using a phase contrast light microscope after staining with a Diff-Quick Stain Kit. The data represents the mean ± SD from triplicate measurements. # P<0.05 versus control; * P<0.05 versus only PMA.