ChemLTP increases SUMO-1 and Ubc9 mRNA.

A) Fluorescent in situ hybridization using cRNA probes for SUMO-1, SUMO-2 and Ubc9 (red) in hippocampal neurons co-stained with the dendritic marker MAP-2 (green). ChemLTP increased levels of SUMO-1 and Ubc9 but not SUMO-2 mRNA in soma and dendrites. Mean fluoresence intensity was quantified in at least 3 independent experiments with 10 neurons per condition and 3 ROIs per neuron using ImageJ. B) ChemLTP increases levels of the mRNA binding protein CPEB1 (red) in hippocampal cultured neurons co-immunostained with MAP2 (green). Three independent experiments with 8 neurons per condition and 4 ROIs per neuron were quantified using JACoP plugin in ImageJ. Statistical significance was determined using ANOVA *p<0.02; **p<0.005. Scale bars – 20 µm.