Characterization of samples purified in a mixture of CHAPS and Fos14 detergents.
2014-06-24T14:52:45Z (GMT) by
<p><i>P. pastoris</i> membranes were solubilized and purified using a two-step purification approach (anti-flag affinity chromatography followed by SEC). <b><i>A</i></b>, Samples were solubilized in a mixture of 0.25% Fos14/0.1% CHAPS and purified in presence of 0.1% Fos14/0.1% CHAPS. <b>1</b>: SDS-PAGE of SEC elution fractions F17 and F22 colored with Coomassie Blue (left) and revealed by anti-Flag immunodetection (right). <b>2</b>: Saturation ligand binding experiments with [<sup>3</sup>H]-melatonin on SEC elution fraction F22. <b>3</b>: Negative staining electron microscopy on SEC elution fraction F22. <b><i>B</i></b>, Samples were solubilized in a mixture of 0.25% Fos14/1% CHAPS, purified in the presence of 0.1% Fos14/0.5% CHAPS, and analyzed by negative staining electron microscopy.</p>